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Xuebiao Nie, Wenjun Liu, Mo Chen, Minmin Liu, Lu Ao
《环境科学与工程前沿(英文)》 2016年 第10卷 第6期 doi: 10.1007/s11783-016-0884-4
关键词: Flow cytometry Escherichia coli Staphylococcusaureus UV CTC SYTO 9
Acetylated HOXB9 at lysine 27 is of differential diagnostic value in patients with pancreatic ductal
Xiaoran Sun, Jiagui Song, Jing Zhang, Jun Zhan, Weigang Fang, Hongquan Zhang
《医学前沿(英文)》 2020年 第14卷 第1期 页码 91-100 doi: 10.1007/s11684-019-0696-6
关键词: HOXB9 AcK27-HOXB9 PDAC
662 A/G gene variation in human tumor necrosis factor receptor superfamily, member 9 (TNFRSF9)
QU Yanchun, YANG Ze, SUN Liang, JI Linong
《医学前沿(英文)》 2008年 第2卷 第3期 页码 283-285 doi: 10.1007/s11684-008-0053-7
关键词: D-HPLC mutation development autoimmune PCR-RFLP candidate
The 9th China Engineering Management Forum Guangzhou Consensus
《工程管理前沿(英文)》 2015年 第2卷 第2期 页码 105-107 doi: 10.15302/J-FEM-2015019
Mobile CRISPR-Cas9 based anti-phage system in E. coli
《化学科学与工程前沿(英文)》 2022年 第16卷 第8期 页码 1281-1289 doi: 10.1007/s11705-022-2141-7
关键词: phage infections anti-phage CRISPR-Cas9 conjugative transfer synthetic biology
REDUCTION OF NON-POINT SOURCE POLLUTION IN THE YONG’AN RIVER BY CONSTRUCTED WETLAND BASED ON 9 YEARS
《农业科学与工程前沿(英文)》 2023年 第10卷 第4期 页码 627-638 doi: 10.15302/J-FASE-2023516
The agricultural and livestock activities surrounding the rivers flowing into the lakes have caused non-point source pollution, leading to excessive amounts of nutrient salts in downstream rivers. Introducing river water into constructed wetlands along river course has proven to be an effective solution for decreasing nitrogen (N) and phosphorus (P) loads. This paper reports 9 years of monitoring the Yong’an River and its surrounding constructed wetlands in the upper reaches of Erhai Lake, located in Yunnan Province, China. This study analyzed the main types of pollutants in the river, and evaluated the removal efficiency of pollutants by the constructed wetlands. The findings indicate that total nitrogen (TN) and nitrate nitrogen (NO3–-N) are the primary pollutants in the Yong’an River, which exhibit variation throughout the year corresponding to the alternating wet and dry seasons. Although constructed wetlands are effective in removing NO3–-N and P, their efficacy in removing ammonium nitrogen (NH4+-N) and organic pollutants is limited. This limitation can be attributed to the lack of timely disposal of aquatic plant residues. This research contributes to the understanding of the potential issues that may arise during the extended use of constructed wetlands and provides solutions to address them.
关键词: inflowing rivers surface-flow constructed wetland nutrients long-term monitoring
Construction of lentiviral vector carrying Rab9 gene and its expression in mouse brain
Youguo HAO, Min ZHANG, Jinzhi XU, Bitao BU, Jiajun WEI
《医学前沿(英文)》 2009年 第3卷 第2期 页码 141-147 doi: 10.1007/s11684-009-0041-6
CRISPR-Cas9 mediated LAG-3 disruption in CAR-T cells
null
《医学前沿(英文)》 2017年 第11卷 第4期 页码 554-562 doi: 10.1007/s11684-017-0543-6
T cells engineered with chimeric antigen receptor (CAR) have been successfully applied to treat advanced refractory B cell malignancy. However, many challenges remain in extending its application toward the treatment of solid tumors. The immunosuppressive nature of tumor microenvironment is considered one of the key factors limiting CAR-T efficacy. One negative regulator of T cell activity is lymphocyte activation gene-3 (LAG-3). We successfully generated LAG-3 knockout T and CAR-T cells with high efficiency using CRISPR-Cas9 mediated gene editing and found that the viability and immune phenotype were not dramatically changed during in vitro culture. LAG-3 knockout CAR-T cells displayed robust antigen-specific antitumor activity in cell culture and in murine xenograft model, which is comparable to standard CAR-T cells. Our study demonstrates an efficient approach to silence immune checkpoint in CAR-T cells via gene editing.
关键词: CAR-T CRISPR-Cas9 LAG-3
Compiling of comprehensive data of human infections with novel influenza A (H7N9) virus
null
《医学前沿(英文)》 2013年 第7卷 第3期 页码 275-276 doi: 10.1007/s11684-013-0285-z
Research progress on human infection with avian influenza H7N9
Xiaoxin Wu, Lanlan Xiao, Lanjuan Li
《医学前沿(英文)》 2020年 第14卷 第1期 页码 8-20 doi: 10.1007/s11684-020-0739-z
Frequency domain a9ctive vibration control of a flexible plate based on neural networks
Jinxin LIU, Xuefeng CHEN, Zhengjia HE
《机械工程前沿(英文)》 2013年 第8卷 第2期 页码 109-117 doi: 10.1007/s11465-013-0252-z
A neural-network (NN)-based active control system was proposed to reduce the low frequency noise radiation of the simply supported flexible plate. Feedback control system was built, in which neural network controller (NNC) and neural network identifier (NNI) were applied. Multi-frequency control in frequency domain was achieved by simulation through the NN-based control systems. A pre-testing experiment of the control system on a real simply supported plate was conducted. The NN-based control algorithm was shown to perform effectively. These works lay a solid foundation for the active vibration control of mechanical structures.
关键词: active vibration control (AVC) neural network (NN) low frequency noise frequency domain control multi-frequency control
阮许平,阮宏伟
《中国工程科学》 2004年 第6卷 第5期 页码 76-78
运用真空电弧炉熔炼合金制备试样,采用三种不同的方法对金属熔体进行处理,试样的X射线衍射晶化相分析结果表明,B2O3和Na2SiO3的混合物与所选组分的熔体发生了反应,不适合作Zr基合金的包裹物;组分为CaF2(45%),AlF3(50%),CaO(5%)以及微量的TiO2的氟盐,由于该净化剂与所选的组分发生了反应,不适合Zr基合金的净化;设备的高真空度、原材料成分的高纯度是促进合金形成非晶态的重要因素。
null
《医学前沿(英文)》 2013年 第7卷 第3期 页码 333-344 doi: 10.1007/s11684-013-0275-1
This study reports the first death caused by a novel avian influenza A (H7N9) virus in Zhejiang Province, China. The patient had chronic hepatitis B and history of exposure to poultry. The patient initially complained of diarrhea and influenza-like symptoms on March 7 and 14 respectively. The disease progressed to severe pneumonia, sustained hypoxia, and coagulation abnormalities. The patient died on March 27 because of respiratory failure, multiple organ failure, and disseminated intravascular coagulation without oseltamivir treatment. This H7N9 virus from Zhejiang is highly similar to isolates obtained from Shanghai, Jiangsu, Anhui, etc. Analysis of hemagglutinin, neuramidinase, and matrix genes indicated that the isolates share the same avian origin, have low virulence, and are sensitive to oseltamivir, but are resistant to adamantine. Only the isolate that caused the fatality exhibited substitution of Q226I in the HA gene, which indicates a potentially enhanced human affinity. The secondary transmission rate was 1.6% (2/125). Only two health workers presented with influenza-like symptoms, and they subsequently tested negative for H7N9 RNA. In conclusion, underlying disease, late diagnosis, and untimely antiviral treatment are possible high-risk factors for infections and death caused by the low-pathogenicity avian influenza A (H7N9). Person-to-person transmission of the H7N9 virus was not detected among close contacts, but such transmission should be investigated in the future. Expanding and enhancing surveillance will help in the early discovery and diagnosis of suspected cases, which will reduce the number of severe cases and deaths.
关键词: avian influenza A (H7N9) virus epidemiology contacts person-to-person transmission
Serum carbohydrate antigen (CA) 19-9 as a prognostic factor in cholangiocarcinoma: A meta-analysis
Shang-Long LIU, Zi-Fang SONG, Qing-Gang HU, Shao-Bo HU, Jun LI, Qi-Chang ZHENG, Duo SHAN,
《医学前沿(英文)》 2010年 第4卷 第4期 页码 457-462 doi: 10.1007/s11684-010-0240-1
关键词: cholangiocarcinoma prognostic factors serum carbohydrate antigen 19-9
Mengmeng ZHAO,Juanjuan QIAN,Jiexiong XIE,Tiantian CUI,Songling FENG,Guoqiang WANG,Ruining WANG,Guihong ZHANG
《农业科学与工程前沿(英文)》 2016年 第3卷 第2期 页码 153-160 doi: 10.15302/J-FASE-2016097
标题 作者 时间 类型 操作
Flow cytometric assessment of the effects of chlorine, chloramine, and UV on bacteria by using nucleic acid stains and 5-cyano-2,3-ditolyltetrazolium chloride
Xuebiao Nie, Wenjun Liu, Mo Chen, Minmin Liu, Lu Ao
期刊论文
Acetylated HOXB9 at lysine 27 is of differential diagnostic value in patients with pancreatic ductal
Xiaoran Sun, Jiagui Song, Jing Zhang, Jun Zhan, Weigang Fang, Hongquan Zhang
期刊论文
662 A/G gene variation in human tumor necrosis factor receptor superfamily, member 9 (TNFRSF9)
QU Yanchun, YANG Ze, SUN Liang, JI Linong
期刊论文
REDUCTION OF NON-POINT SOURCE POLLUTION IN THE YONG’AN RIVER BY CONSTRUCTED WETLAND BASED ON 9 YEARS
期刊论文
Construction of lentiviral vector carrying Rab9 gene and its expression in mouse brain
Youguo HAO, Min ZHANG, Jinzhi XU, Bitao BU, Jiajun WEI
期刊论文
Research progress on human infection with avian influenza H7N9
Xiaoxin Wu, Lanlan Xiao, Lanjuan Li
期刊论文
Frequency domain a9ctive vibration control of a flexible plate based on neural networks
Jinxin LIU, Xuefeng CHEN, Zhengjia HE
期刊论文
The first avian influenza A (H7N9) viral infection in humans in Zhejiang Province, China: a death report
null
期刊论文
Serum carbohydrate antigen (CA) 19-9 as a prognostic factor in cholangiocarcinoma: A meta-analysis
Shang-Long LIU, Zi-Fang SONG, Qing-Gang HU, Shao-Bo HU, Jun LI, Qi-Chang ZHENG, Duo SHAN,
期刊论文